Genotype

GENOTYPE TEST

AIM:- To separate and identify the hemoglobin genotype of an individual.

APPARATUS: - Blood sample, As control, cellulose acetate paper, electrophoresis machine, Absorbent paper, little cover slip, water, micro pipette.

PRINCIPLE:- Rapid movement or migration of hemoglobin of normal cell in an electric field.

PROCEDURE:-        

• Wet an acetate paper with buffer solution; dry it in between folded absorbent paper.

• Place a drop each of the patient blood and the control as sample on a white tile.

• Pipette one or two drops of water on each of the dropped blood and mix thoroughly to lyses the red blood cell.

• With the edge of a tiny cover slip, dip into the mixed blood and make bands on the acetate paper close to the edge on a straight line.

• Place the paper in the electrophoresis machine with buffer water inside the machine and cover.

OBSERVATION:-

Bands separate after a few minutes. The component of the blood separates by chromatographic movement on the acetate paper.

RESULT;-

AS       -           Two bands, one on top and the other below.

AA      -           Two bands both above.

SS        -           Two bands both below.